Effect of Phospholipase C-gamma1 Overexpression on the Protein Level of Waf1, PCNA, and Cyclin B1 Following Ultraviolet C Irradiation / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 427-432, 2001.
Artigo
em Coreano
| WPRIM
| ID: wpr-196396
ABSTRACT
PURPOSE:
It has been demonstrated that PLC-gamma1 is overexpressed in many tumor cells, and that overexpression of Phospholipase C (PLC)-gamma1 is associated with tumor progression. In order to understand the effect of the PLC-gamma1 overexpression on the regulation of cell cycle regulators following DNA damage, we analyzed the expression level of PCNA, cyclin B1, and p21 Waf1 after ultraviolet C (UVC) irradiation in PLC-gamma1-transfected PC12 cells. MATERIALS ANDMETHODS:
PC12 and 3Y1 cells, transfected with empty vector or rat PLC-gamma1 cDNA, were used for this study. Following UVC irradiation, cell cycle progression was analyzed by flow cytometry and protein expression was detected by Western blotting.RESULTS:
Waf1 protein was markedly down-regulated, whereas PCNA and cyclin B1 was up-regulated in PLC-gamma1 overexpressed-cells as compared to the vector transfected-cells. When the cells were irradiated with UVC, PCNA was slightly increased within 3-hours of the UV irradiation and then was markedly decreased in Vector/ PC12 cells, while it remained high until 37 hour after UVC in PLC-gamma1/PC12 cells. In contrast, cyclin B1 was gradually decreased following UVC irradiation in both cells.CONCLUSION:
The overexpression of PLC-gamma1 affects the expression level of PCNA after UVC irradiation. We proposed that the overexpression of PLC-gamma1 may contribute to the UV-induced genomic instability by up-regulating the expression of PCNA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfolipases Tipo C
/
Fosfolipases
/
Dano ao DNA
/
Ciclo Celular
/
Western Blotting
/
Células PC12
/
Ciclinas
/
DNA Complementar
/
Antígeno Nuclear de Célula em Proliferação
/
Instabilidade Genômica
Limite:
Animais
Idioma:
Coreano
Revista:
Cancer Research and Treatment
Ano de publicação:
2001
Tipo de documento:
Artigo
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