A Model of Glial Scarring Analogous to the Environment of a Traumatically Injured Spinal Cord Using Kainate
Annals of Rehabilitation Medicine
;
: 757-768, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-196576
ABSTRACT
OBJECTIVE:
To develop an in vitro model analogous to the environment of traumatic spinal cord injury (SCI), the authors evaluated change of astrogliosis following treatments with kainate and/or scratch, and degree of neurite outgrowth after treatment with a kainate inhibitor.METHODS:
Astrocytes were obtained from the rat spinal cord. Then, 99% of the cells were confirmed to be GFAP-positive astrocytes. For chemical injury, the cells were treated with kainate at different concentrations (10, 50 or 100 µM). For mechanical injury, two kinds of uniform scratches were made using a plastic pipette tip by removing strips of cells. For combined injury (S/K), scratch and kainate were provided. Cord neurons from rat embryos were plated onto culture plates immediately after the three kinds of injuries and some cultures were treated with a kainate inhibitor.RESULTS:
Astro-gliosis (glial fibrillary acidic protein [GFAP], vimentin, chondroitin sulfate proteoglycan [CSPG], rho-associated protein kinase [ROCK], and ephrin type-A receptor 4 [EphA4]) was most prominent after treatment with 50 µM kainate and extensive scratch injury in terms of single arm (p<0.001) and in the S/K-induced injury model in view of single or combination (p<0.001). Neurite outgrowth in the seeded spinal cord (β-III tubulin) was the least in the S/K-induced injury model (p<0.001) and this inhibition was reversed by the kainate inhibitor (p<0.001).CONCLUSION:
The current in vitro model combining scratch and kainate induced glial scarring and inhibitory molecules and restricted neurite outgrowth very strongly than either the mechanically or chemically-induced injury model; hence, it may be a useful tool for research on SCI.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Braço
/
Plásticos
/
Proteínas Quinases
/
Proteoglicanas de Sulfatos de Condroitina
/
Medula Espinal
/
Traumatismos da Medula Espinal
/
Vimentina
/
Técnicas In Vitro
/
Neuroglia
/
Astrócitos
Limite:
Animais
Idioma:
Inglês
Revista:
Annals of Rehabilitation Medicine
Ano de publicação:
2016
Tipo de documento:
Artigo
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