The interaction of intrathecal neostigmine and N(6)-cyclohexyladenosine on anti-allodynic effects in rats with a nerve ligation injury / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 39-44, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-196641
ABSTRACT
BACKGROUND:
Nerve ligation injury in rats produces a pain syndrome that includes mechanical allodynia. Intrathecal administration of cholinesterase inhibitors or adenosine receptor agonists have anti-allodynic effects in this model. Therefore, we tested the interaction between intrathecal neostigmine and N(6)-cyclohexyladenosine (CHA) in a rat behavioral model of neuropathic pain.METHODS:
Male Sprague-Dawley rats were prepared with tight ligation of the spinal nerves for producing allodynia and with a lumbar intrathecal catheter for drug administration. Allodynia thresholds for hindpaw withdrawal against mechanical stimuli were assessed and converted to percent maximal possible effect. Neostigmine (0.3-10 microgram) and CHA (0.03-3 microgram) were administered to obtain the dose-response curves and the 50% effective dose (ED(50)). Equal fractions (1/2, 1/4 and 1/8 ED(50)s) of the two drugs were administered to establish the ED(50) of neostigmine-CHA combination. Side effects were also assessed. The drug interaction was evaluated by isobolographic and fractional analyses.RESULTS:
Neostigmine, CHA, and the neostigmine-CHA combination dose-dependently produced anti-allodynia effects. Side effects such as sedation and motor weakness were similar in the three groups. In the isobolographic analysis, the experimental ED(50) for the combination of neostigmine-CHA lay far below and to the left of the theoretical additive line. Fractional analysis indicated that the total combination fraction of the two drugs was 0.39.CONCLUSIONS:
Intrathecal co-administration of neostigmine and CHA showed a synergistic anti-allodynia effect.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Nervos Espinhais
/
Injeções Espinhais
/
Adenosina
/
Inibidores da Colinesterase
/
Ratos Sprague-Dawley
/
Interações Medicamentosas
/
Agonistas do Receptor Purinérgico P1
/
Catéteres
/
Hiperalgesia
/
Ligadura
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Korean Journal of Anesthesiology
Ano de publicação:
2010
Tipo de documento:
Artigo
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