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Effect of Heat Shock Protein 72 Expression on Etoposide-induced Cell Death of Rat Retinal Ganglion Cells
Korean Journal of Ophthalmology ; : 48-51, 2013.
Artigo em Inglês | WPRIM | ID: wpr-19707
ABSTRACT

PURPOSE:

To assess whether the expression of heat shock protein 72 (Hsp72) protects rat retinal ganglion cells (RGC-5) from apoptotic cell death.

METHODS:

Hsp72 expression in RGC-5 cells transduced with replication-deficient recombinant adenovirus was analyzed by Western blot analysis and immunofluorescence. The effect of Hsp72 expression on etoposide-induced apoptotic cell death was examined by microscopic analysis and confirmed by cell proliferation assay.

RESULTS:

Western blot analysis and immunofluorescence clearly showed adenovirus-mediated Hsp72 expression in RGC-5 cells. Treatment with etoposide resulted in the death of a proportion of the cells by apoptosis. However, this apoptotic cell death was significantly reduced in cells expressing Hsp72, with the reduction in cell death correlating to the level of Hsp72 expression.

CONCLUSIONS:

Over-expression of Hsp72 alone is sufficient to rescue neuronal cells from apoptotic cell death, suggesting that fine-tuning its expression may be an effective neuroprotective approach in retinal degenerative disease.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Degeneração Retiniana / Células Ganglionares da Retina / DNA / Imuno-Histoquímica / Sobrevivência Celular / Células Cultivadas / Regulação da Expressão Gênica / Western Blotting / Morte Celular / Modelos Animais de Doenças Limite: Animais Idioma: Inglês Revista: Korean Journal of Ophthalmology Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Degeneração Retiniana / Células Ganglionares da Retina / DNA / Imuno-Histoquímica / Sobrevivência Celular / Células Cultivadas / Regulação da Expressão Gênica / Western Blotting / Morte Celular / Modelos Animais de Doenças Limite: Animais Idioma: Inglês Revista: Korean Journal of Ophthalmology Ano de publicação: 2013 Tipo de documento: Artigo