Cellular uptake of magnetic nanoparticle is mediated through energydependent endocytosis in A549 cells
Journal of Veterinary Science
;
: 321-326, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-197261
ABSTRACT
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which the lung cancer cells uptake the magnetic nanoparticles. Lung cells were chosen for this study because inhalation is the most likely route of exposure and lung cancer cells were also found to uptake magnetic nanoparticles rapidly in preliminary experiments. The lung cells were pretreated with different metabolic inhibitors. Our results revealed that low temperature disturbed the uptake of magnetic nanoparticles into the cells. Metabolic inhibitors also prevented the delivery of the materials into cells. Use of TEM clearly demonstrated that uptake of the nanoparticles was mediated through endosomes. Taken together, our results demonstrate that magnetic nanoparticles can be internalized into the cells through an energy-dependent endosomal-lysosomal mechanism.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Endossomos
/
Sacarose
/
Temperatura
/
Materiais Biocompatíveis
/
Sistemas de Liberação de Medicamentos
/
Microscopia Confocal
/
Macrolídeos
/
Azida Sódica
/
Linhagem Celular Tumoral
/
Microscopia Eletrônica de Transmissão
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2006
Tipo de documento:
Artigo
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