NF-kappaB Binding Activity and Cyclooxygenase-2 Expression in Persistent betaCCI(4)-Treated Rat Liver Injury
Journal of Korean Medical Science
;
: 193-200, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-197892
ABSTRACT
The involvement of NF-kappaB binding activity is known to be important in the mechanism of acute liver injury and in the induction of cyclooxygenase (COX-2). This study was performed to evaluate NF-kappaB binding activity and the expression of COX-2 in chronic liver injury induced by carbon tetrachloride (betaCCI(4)). Liver tissues from Sprague - Dawley rats were collected at 1, 3, 5, and 7th week after intraperitoneal injection of 0.1 mL of betaCCI(4)/100 g body weight twice a week. Reactive oxy-gen species (ROS) were measured in the postmitochondrial fraction by dichlorofluorescein formation with a fluorescent probe. An electrophoretic mobility shift assay was performed for NF-kappaB binding activity. Western blot was performed to measure the level of COX-1, COX-2, p65, p50, and I B proteins. ROS and NF-kappaB activity increased during the CCl4-induced chronic liver injury. The expression of nuclear p65 protein and p50 protein increased compared with that of the control, while the cytoplasmic I B protein decreased as the inflammation persisted. The expression of COX-2 in betaCCI(4)-treated rat liver increased compared with that of the control. It could be suggested that ROS produced by betaCCI(4) treatment increased NF-kappaB binding activity and thereby COX-2 expression, and these might be implicated in the progress of chronic liver damage.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ligação Proteica
/
Transporte Biológico
/
Tetracloreto de Carbono
/
Intoxicação por Tetracloreto de Carbono
/
Núcleo Celular
/
NF-kappa B
/
Prostaglandina-Endoperóxido Sintases
/
Espécies Reativas de Oxigênio
/
Ratos Sprague-Dawley
/
Proteínas I-kappa B
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Korean Medical Science
Ano de publicação:
2002
Tipo de documento:
Artigo
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