Natural occurrence of Mycobacterium as an endosymbiont of Acanthamoeba isolated from a contact lens storage case
The Korean Journal of Parasitology
;
: 11-18, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-198437
ABSTRACT
Recent in vitro studies have revealed that a certain Mycobacterium can survive and multiply within freeliving amoebae. It is believed that protozoans function as host cells for the intracellular replication and evasion of Mycobacterium spp. under harmful conditions. In this study, we describe the isolation and characterization of a bacterium naturally observed within an amoeba isolate acquired from a contact lens storage case. The bacterium multiplied within Acanthamoeba, but exerted no cytopathic effects on the amoeba during a 6-year amoebic culture. Trasnmission electron microscopy showed that the bacteria were randomly distributed within the cytoplasm of trophozoites and cysts of Acanthamoeba. On the basis of the results of 18S rRNA gene analysis, the amoeba was identified as A. lugdunensis. A 16S rRNA gene analysis placed this bacterium within the genus Mycobacterium. The bacterium evidenced positive reactivity for acid-fast and fluorescent acid-fast stains. The bacterium was capable of growth on the Middlebrook 7H11-Mycobacterium-specific agar. The identification and characterization of bacterial endosymbionts of free-living protozoa bears significant implications for our understanding of the ecology and the identification of other atypical mycobacterial pathogens.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Simbiose
/
Polimorfismo de Fragmento de Restrição
/
DNA Mitocondrial
/
Acanthamoeba
/
RNA Ribossômico 16S
/
RNA Ribossômico 18S
/
Sequência de Bases
/
Soluções para Lentes de Contato
/
Lentes de Contato
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2007
Tipo de documento:
Artigo
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