Silymarin Inhibits Cytokine-Stimulated Pancreatic Beta Cells by Blocking the ERK1/2 Pathway
Biomolecules & Therapeutics
; : 282-287, 2014.
Article
em En
| WPRIM
| ID: wpr-199235
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WPRO
ABSTRACT
We show that silymarin, a polyphenolic flavonoid isolated from milk thistle (Silybum marianum), inhibits cytokine mixture (CM: TNF-alpha, IFN-gamma, and IL-1beta)-induced production of nitric oxide (NO) in the pancreatic beta cell line MIN6N8a. Immunostaining and Western blot analysis showed that silymarin inhibits iNOS gene expression. RT-PCR showed that silymarin inhibits iNOS gene expression in a dose-dependent manner. We also showed that silymarin inhibits extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) phosphorylation. A MEK1 inhibitor abrogated CM-induced nitrite production, similar to silymarin. Treatment of MIN6N8a cells with silymarin also inhibited CM-stimulated activation of NF-kappaB, which is important for iNOS transcription. Collectively, we demonstrate that silymarin inhibits NO production in pancreatic beta cells, and silymarin may represent a useful anti-diabetic agent.
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WPRIM
Assunto principal:
Fosforilação
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Silimarina
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Expressão Gênica
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Western Blotting
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NF-kappa B
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Fator de Necrose Tumoral alfa
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Silybum marianum
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Células Secretoras de Insulina
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Óxido Nítrico
Idioma:
En
Revista:
Biomolecules & Therapeutics
Ano de publicação:
2014
Tipo de documento:
Article