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Peroxiredoxin-3 Is Involved in Bactericidal Activity through the Regulation of Mitochondrial Reactive Oxygen Species
Immune Network ; : 373-380, 2016.
Artigo em Inglês | WPRIM | ID: wpr-199704
ABSTRACT
Peroxiredoxin-3 (Prdx3) is a mitochondrial protein of the thioredoxin family of antioxidant peroxidases and is the principal peroxidase responsible for metabolizing mitochondrial hydrogen peroxide. Recent reports have shown that mitochondrial reactive oxygen species (mROS) contribute to macrophage-mediated bactericidal activity in response to Toll-like receptors. Herein, we investigated the functional effect of Prdx3 in bactericidal activity. The mitochondrial localization of Prdx3 in HEK293T cells was confirmed by cell fractionation and confocal microscopy analyses. To investigate the functional role of Prdx3 in bactericidal activity, Prdx3-knockdown (Prdx3(KD)) THP-1 cells were generated. The mROS levels in Prdx3(KD) THP-1 cells were significantly higher than those in control THP-1 cells. Moreover, the mROS levels were markedly increased in response to lipopolysaccharide. Notably, the Salmonella enterica serovar Typhimurium infection assay revealed that the Prdx3(KD) THP-1 cells were significantly resistant to S. Typhimurium infection, as compared with control THP-1 cells. Taken together, these results indicate that Prdx3 is functionally important in bactericidal activity through the regulation of mROS.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Peroxidases / Tiorredoxinas / Fracionamento Celular / Lipopolissacarídeos / Espécies Reativas de Oxigênio / Peroxidase / Microscopia Confocal / Salmonella enterica / Proteínas Mitocondriais / Receptores Toll-Like Limite: Humanos Idioma: Inglês Revista: Immune Network Ano de publicação: 2016 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Peroxidases / Tiorredoxinas / Fracionamento Celular / Lipopolissacarídeos / Espécies Reativas de Oxigênio / Peroxidase / Microscopia Confocal / Salmonella enterica / Proteínas Mitocondriais / Receptores Toll-Like Limite: Humanos Idioma: Inglês Revista: Immune Network Ano de publicação: 2016 Tipo de documento: Artigo