Mechanisms Underlying Trabecular Meshwork Cell Death Caused by Mutant Myocilin Expression
Journal of the Korean Ophthalmological Society
;
: 1507-1513, 2011.
Artigo
em Coreano
| WPRIM
| ID: wpr-200322
ABSTRACT
PURPOSE:
To determine whether the expression of mutant myocilin can lead to death of human trabecular meshwork (HTM) cells and to determine whether the mechanism by which this occurs is apoptosis.METHODS:
HTM cells were transduced with a recombinant adenovirus expressing human mutant (Q368X) myocilin. The apoptotic death of HTM cells caused by expression of mutant myocilin was examined using a cell proliferation assay, flow cytometry, Western blot analysis, and immunocytochemistry.RESULTS:
It appeared that the expression of mutant myocilin itself was not sufficient to cause HTM cell death. Furthermore, the expression of mutant myocilin did not lead to apoptosis of HTM cells although it did elicit a protein unfolding response.CONCLUSIONS:
Our data suggest that the mechanism of myocilin glaucoma is not apoptotic death of HTM cells caused by mutant myocilin expression, and that the actual mechanism remains unknown.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Malha Trabecular
/
Glicoproteínas
/
Adenoviridae
/
Glaucoma
/
Western Blotting
/
Morte Celular
/
Apoptose
/
Proteínas do Citoesqueleto
/
Proliferação de Células
/
Proteínas do Olho
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2011
Tipo de documento:
Artigo
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