Development of Competitive ELISA for Detection of Avian Metapneumovirus Antibodies in Chicken
Journal of Bacteriology and Virology
;
: 131-143, 2010.
Artigo
em Coreano
| WPRIM
| ID: wpr-200688
ABSTRACT
Avian metapneumovirus (aMPV) causes an acute and highly contagious upper respiratory tract infection in turkeys and chickens. In this study, a competitive ELISA (C-ELISA) was developed for the detection of antibodies to aMPV in chicken sera and/or their egg yolks. This assay is based on the competitive binding of monoclonal antibody with serum antibodies to recombinant aMPV N protein expressed by a recombinant baculovirus. The C-ELISA showed specificity and sensitivity of 100% and 98.0%, respectively, when compared to the virus neutralization test. In specific pathogen-free chickens experimentally infected with aMPV SC1509 strain, the C-ELISA started to detect antibodies to aMPV as early as 5 days post infection from birds infected with aMPV, while a commercial ELISA kit detected first 10 days post infection. The C-ELISA was similar or superior to a commercial ELISA kit when serum and egg yolk samples collected from chickens on six outbreak farms were tested for diagnosis. The C-ELISA developed in the present work provides a short turnaround time and can be a useful diagnostic and screening tool for aMPV infection in the field.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Infecções Respiratórias
/
Entorses e Distensões
/
Perus
/
Vírus
/
Ligação Competitiva
/
Aves
/
Ensaio de Imunoadsorção Enzimática
/
Testes de Neutralização
/
Galinhas
/
Programas de Rastreamento
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Coreano
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2010
Tipo de documento:
Artigo
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