Identification of Helicobacter pylori Strain 51 Major Outer Membrane Proteins by Quadrupole Time of Flight Mass Spectrometry
Journal of Bacteriology and Virology
;
: 103-109, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-200691
ABSTRACT
As part of an initial inquiry into the function of the outer membrane proteins (OMPs) of Helicobacter pylori Korean strain 51, we have conducted an extensive proteome analysis via quadrupole time of flight (Q-TOF) mass spectrometry (MS). Fifty one OMPs of H. pylori were purified using sarcosine and resolved via two-dimensional electrophoresis with immobilized pH gradient strips. The most abundant proteins were observed in the alkaline pI regions (6.0~11.0) at molecular masses between 10~100 KDa. Here, 15 spots were identified, representing 9 types of genes (KHP0852, KHP0853, KHP1353, KHP1017, KHP0172, KHP0076, KHP0617, KHP1069, KHP0614) from the sarcosin-insoluble fraction of H. pylori 51. These may be employed in the characterization of the OMPs of H. pylori 51, which will help to identify new potential target proteins for vaccine development and drug therapy.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sarcosina
/
Espectrometria de Massas
/
Entorses e Distensões
/
Proteínas
/
Helicobacter pylori
/
Helicobacter
/
Força Próton-Motriz
/
Proteoma
/
Eletroforese
/
Proteínas de Membrana
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2010
Tipo de documento:
Artigo
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