Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model / 부인종양
Journal of Gynecologic Oncology
; : 328-333, 2014.
Article
em En
| WPRIM
| ID: wpr-202217
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE: To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment. METHODS: Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 microg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4degrees C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30degrees C for hormone assays. RESULTS: All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Mullerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group. CONCLUSION: G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Biomarcadores
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Fator Estimulador de Colônias de Granulócitos
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Cisplatino
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Insuficiência Ovariana Primária
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Ratos Sprague-Dawley
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Modelos Animais de Doenças
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Avaliação Pré-Clínica de Medicamentos
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Hormônio Antimülleriano
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Preservação da Fertilidade
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Folículo Ovariano
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Journal of Gynecologic Oncology
Ano de publicação:
2014
Tipo de documento:
Article