Administration of Phytoceramide Enhances Memory and Upregulates the Expression of pCREB and BDNF in Hippocampus of Mice
Biomolecules & Therapeutics
; : 229-233, 2013.
Article
em En
| WPRIM
| ID: wpr-202359
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed at investigating the possible effects of phytoceramide (Pcer) on learning and memory and their underlying mechanisms. Phytoceramide was orally administered to ICR mice for 7 days. Memory performances were assessed using the passive avoidance test and Y-maze task. The expressions of phosphorylated cAMP response element binding protein (pCREB), brain-derived neurotrophic factor (BDNF) were measured with immunoblot. The incorporation of 5-bromo-2-deoxyuridine (BrdU) in hippocampal regions was investigated by using immunohistochemical methods. Treatment of Pcer enhanced cognitive performances in the passive avoidance test and Y-maze task. Immunoblotting studies revealed that the phosphorylated CREB and BDNF were significantly increased on hippocampus in the Pcer-treated mice. Immunohistochemical studies showed that the number of immunopositive cells to BrdU was significantly increased in the hippocampal dentate gyrus regions after Pcer-treatment for 7 days. These results suggest that Pcer contribute to enhancing memory and BDNF expression and it could be secondary to the elevation of neurogenesis.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Bromodesoxiuridina
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Immunoblotting
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Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico
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Giro Denteado
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Fator Neurotrófico Derivado do Encéfalo
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Neurogênese
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Hipocampo
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Aprendizagem
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Memória
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Métodos
Limite:
Animals
Idioma:
En
Revista:
Biomolecules & Therapeutics
Ano de publicação:
2013
Tipo de documento:
Article