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Multiple transcripts of anoctamin genes expressed in the mouse submandibular salivary gland
Article em En | WPRIM | ID: wpr-202423
Biblioteca responsável: WPRO
ABSTRACT
PURPOSE: Salivary fluid formation is primarily driven by Ca2+-activated, apical efflux of chloride into the lumen of the salivary acinus. The anoctamin1 protein is an anion channel with properties resembling the endogenous calcium-activated chloride channels. In order to better understand the role of anoctamin proteins in salivary exocrine secretion, the expression of the ten members of the anoctamin gene family in the mouse submandibular gland was studied. METHODS: Total RNA extracted from mouse submandibular salivary glands was reverse transcribed using primer pairs to amplify the full-length coding regions of each anoctamin gene and was subcloned into plasmid vectors for DNA sequencing. Alternative splice variants were also screened by polymerase chain reaction using primer pairs that amplified six overlapping regions of the complementary DNA of each anoctamin gene, spanning multiple exons. RESULTS: Multiple anoctamin transcripts were found in the mouse submandibular salivary gland, including full-length transcripts of anoctamin1, anoctamin3, anoctamin4, anoctamin5, anoctamin6, anoctamin9, and anoctamin10. Exon-skipping splicing in the N-terminal exons of the anoctamins1, anoctamin5, and anoctamin6 genes resulted in multiple alternative splice variants. No expression of anoctamin2, anoctamin7, or anoctamin8 was found. CONCLUSIONS: The predominant anoctamin transcript expressed in the mouse submandibular gland is anoctamin1ac. The chloride channel protein produced by anoctamin1ac is likely responsible for the Ca2+-activated chloride efflux, which is the rate-limiting step in salivary exocrine secretion.
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Texto completo: 1 Índice: WPRIM Assunto principal: Plasmídeos / Glândulas Salivares / Glândula Submandibular / RNA / Reação em Cadeia da Polimerase / Éxons / Análise de Sequência de DNA / Processamento Alternativo / DNA Complementar / Canais de Cloreto Limite: Animals / Humans Idioma: En Revista: Journal of Periodontal & Implant Science Ano de publicação: 2015 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Plasmídeos / Glândulas Salivares / Glândula Submandibular / RNA / Reação em Cadeia da Polimerase / Éxons / Análise de Sequência de DNA / Processamento Alternativo / DNA Complementar / Canais de Cloreto Limite: Animals / Humans Idioma: En Revista: Journal of Periodontal & Implant Science Ano de publicação: 2015 Tipo de documento: Article