LIN28B confers radio-resistance through the posttranscriptional control of KRAS
Experimental & Molecular Medicine
;
: 912-918, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-202556
ABSTRACT
To screen the differentially expressed microRNAs related to radio-resistance, we compared the microRNA profiles of lung cancer cells with different responses to ionizing radiation (IR). Of 328 microRNAs in microarray, 27 microRNAs were differentially expressed in NCI-H460 (H460) and NCI-H1299 (H1299) cells. Among them, let-7g was down-regulated in radio-resistant H1299 cells, and the level of let-7g was higher in radio-sensitive cells like Caski, H460, and ME180 in qRT-PCR analysis than in radio-resistant cells like A549, H1299, DLD1, and HeLa. Over-expression of let-7g in H1299 cells could suppress the translation of KRAS, and increase the sensitivity to IR. When we knockdown the expression of LIN28B, an upstream regulator of let-7g, the level of mature let-7g was increased in H1299 cells and the sensitivity to IR was also enhanced in LIN28B knockdown cells. From these data, we suggest that LIN28B plays an important role in radiation responses of lung cancer cells through inhibiting let-7g processing and increasing translation of KRAS.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tolerância a Radiação
/
Regulação Neoplásica da Expressão Gênica
/
Proteínas Proto-Oncogênicas
/
Proteínas ras
/
Perfilação da Expressão Gênica
/
MicroRNAs
/
Linhagem Celular Tumoral
/
Proteínas de Ligação a DNA
/
Neoplasias Pulmonares
Limite:
Humanos
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2009
Tipo de documento:
Artigo
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