Cellular immune response following pre-exposure and postexposure rabies vaccination by intradermal and intramuscular routes
Clinical and Experimental Vaccine Research
;
: 68-74, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-203149
ABSTRACT
PURPOSE:
Immunization against rabies in humans induces protective neutralizing antibodies; however, the induction of type 1 or type 2 cytokine mediated cellular immune responses following rabies vaccination is not understood. Hence, the present study investigated cellular cytokine responses in vaccinated individuals. MATERIALS ANDMETHODS:
The study groups included healthy rabies antigen naive controls (n=10), individuals who received intradermal primary (n=10) or booster pre-exposure vaccination (n=20) and subjects who received postexposure rabies vaccination either by intradermal (n=18) or intramuscular (n=20) routes. The antigen specific cellular responses were analyzed by stimulating peripheral blood mononuclear cells with a rabies vaccine antigen in the interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) enzyme-linked immunospot (ELISpot) assay. These responses were compared to the rabies virus neutralizing antibody (RVNA) titers that were measured by rapid fluorescent focus inhibition test.RESULTS:
We observed that cellular and humoral immune responses to primary intradermal rabies vaccination could be greatly enhanced by a booster vaccine; and both type 1 and type 2 cytokine responses were significantly elevated. The magnitude of type 1 and type 2 cytokine responses did not differ significantly among the intramuscular and intradermal routes of postexposure vaccination. The number of cells producing IFN-gamma and IL-4 correlated significantly with the levels of RVNA.CONCLUSION:
Both type 1 and type 2 cellular cytokine responses are strongly induced after rabies vaccination and directly correlate with levels of RVNA titers. The neutralizing antibody as well as the type 1 and type 2 cytokine responses may be important for vaccine induced protective responses against rabies.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Raiva
/
Vírus da Raiva
/
Vacina Antirrábica
/
Interleucina-4
/
Imunização
/
Interferon gama
/
Vacinação
/
Anticorpos Neutralizantes
/
Imunidade Humoral
/
Imunidade Celular
Limite:
Humanos
Idioma:
Inglês
Revista:
Clinical and Experimental Vaccine Research
Ano de publicação:
2015
Tipo de documento:
Artigo
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