Effects of Insulin-Like Growth Factor-1 on Proliferation of Human Corpus Cavernosal Smooth Muscle Cells / 대한남성과학회지
Korean Journal of Andrology
;
: 18-22, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-203313
ABSTRACT
PURPOSE:
Insulin-like growth factor-1 (IGF-1) promotes the proliferation and migration of penile cavernous smooth muscle cells in the rat. The goals of this study were to evaluate the expression of IGF-1 and IGF-1 receptor (IGF-1R) in human corpus cavernosal smooth muscle cells (HCCSMCs) and to investigate the effect of IGF-1 on the proliferation of these cells in vitro. MATERIALS ANDMETHODS:
The HCCSMCs were cultured from five impotent patients. The smooth muscle cells were identified by immunofluorescent stain. Total RNA was extracted, and IGF-1 gene expression was determined by RT-PCR. The IGF-1R immunoreactivity was examined by fluorescent Immunocytochemistry. Cell growth was assessed with a novel proliferation assay based on bioreduction of the fluorescent dye, Alamar blue.RESULTS:
Endogenous IGF-1 mRNA expression was detected by RT-PCR analysis. The HCCSMCs were positive for IGF-IR. The proliferation of HCCSMCs increased with the dose in response IGF-1 in the culture medium in concentrations up to 100 ng/ml.CONCLUSIONS:
Both IGF-1 and IGF-1R are expressed endogenously in human corpus cavernosal smooth muscle cells. IGF-1 promotes the proliferation of these cells in vitro.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA
/
RNA Mensageiro
/
Fator de Crescimento Insulin-Like I
/
Imuno-Histoquímica
/
Expressão Gênica
/
Receptor IGF Tipo 1
/
Miócitos de Músculo Liso
/
Músculo Liso
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Andrology
Ano de publicação:
2003
Tipo de documento:
Artigo
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