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Effects of Insulin-Like Growth Factor-1 on Proliferation of Human Corpus Cavernosal Smooth Muscle Cells / 대한남성과학회지
Korean Journal of Andrology ; : 18-22, 2003.
Artigo em Coreano | WPRIM | ID: wpr-203313
ABSTRACT

PURPOSE:

Insulin-like growth factor-1 (IGF-1) promotes the proliferation and migration of penile cavernous smooth muscle cells in the rat. The goals of this study were to evaluate the expression of IGF-1 and IGF-1 receptor (IGF-1R) in human corpus cavernosal smooth muscle cells (HCCSMCs) and to investigate the effect of IGF-1 on the proliferation of these cells in vitro. MATERIALS AND

METHODS:

The HCCSMCs were cultured from five impotent patients. The smooth muscle cells were identified by immunofluorescent stain. Total RNA was extracted, and IGF-1 gene expression was determined by RT-PCR. The IGF-1R immunoreactivity was examined by fluorescent Immunocytochemistry. Cell growth was assessed with a novel proliferation assay based on bioreduction of the fluorescent dye, Alamar blue.

RESULTS:

Endogenous IGF-1 mRNA expression was detected by RT-PCR analysis. The HCCSMCs were positive for IGF-IR. The proliferation of HCCSMCs increased with the dose in response IGF-1 in the culture medium in concentrations up to 100 ng/ml.

CONCLUSIONS:

Both IGF-1 and IGF-1R are expressed endogenously in human corpus cavernosal smooth muscle cells. IGF-1 promotes the proliferation of these cells in vitro.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: RNA / RNA Mensageiro / Fator de Crescimento Insulin-Like I / Imuno-Histoquímica / Expressão Gênica / Receptor IGF Tipo 1 / Miócitos de Músculo Liso / Músculo Liso Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Coreano Revista: Korean Journal of Andrology Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: RNA / RNA Mensageiro / Fator de Crescimento Insulin-Like I / Imuno-Histoquímica / Expressão Gênica / Receptor IGF Tipo 1 / Miócitos de Músculo Liso / Músculo Liso Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Coreano Revista: Korean Journal of Andrology Ano de publicação: 2003 Tipo de documento: Artigo