A Comparative Study of Gene Microarray between Normal and Diabetic Keratocyte in Rat Cornea
Journal of the Korean Ophthalmological Society
;
: 1629-1638, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-20346
ABSTRACT
PURPOSE:
To differentiate the genes that are up-regulated or down-regulated and the cellular morphology in the corneal stromal cells between normal and diabetic rat.METHODS:
After isolation with trizol (GIBCO, NY, USA), labeling and purification of complementary DNA(cDNA) probes were carried out using Atlas(TM) Glass Microarrays 1.0.RESULTS:
Autoradiographic analysis showed that 35 genes were up-regulated and 8 genes were down-regulated by Atlas Image(TM) Software. Up-regulated genes were as calcium/calmodulin dependent protein kinase I, proteasome 26S subunit 1, prostaglandin F receptor. Down-regulated genes were aquaporin 8, cytochrome c oxidase polypeptide Vb, glypican 1.CONCLUSIONS:
There were many differential genes in hormone receptor, voltage gated ion channels, oncogenes and tumor suppressors, G protein between normal and diabetic keratocyte of rat.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oncogenes
/
Proteínas Quinases
/
Células Estromais
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Proteínas de Ligação ao GTP
/
Córnea
/
Complexo de Endopeptidases do Proteassoma
/
Glipicanas
/
Vidro
/
Canais Iônicos
Limite:
Animais
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2003
Tipo de documento:
Artigo
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