Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration
Journal of Dental Anesthesia and Pain Medicine
;
: 191-198, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-203997
ABSTRACT
BACKGROUND:
For peripheral nerve regeneration, recent attentions have been paid to the nerve conduits made by tissue-engineering technique. Three major elements of tissue-engineering are cells, molecules, and scaffolds.METHODS:
In this study, the attachments of nerve cells, including Schwann cells, on the nerve conduit and the effects of both growth factor and adhesion molecule on these attachments were investigated.RESULTS:
The attachment of rapidly-proliferating cells, C6 cells and HS683 cells, on nerve conduit was better than that of slowly-proliferating cells, PC12 cells and Schwann cells, however, the treatment of nerve growth factor improved the attachment of slowly-proliferating cells. In addition, the attachment of Schwann cells on nerve conduit coated with fibronectin was as good as that of Schwann cells treated with glial cell line-derived neurotrophic factor (GDNF).CONCLUSIONS:
Growth factor changes nerve cell morphology and affects cell cycle time. And nerve growth factor or fibronectin treatment is indispensable for Schwann cell to be used for implantation in artificial nerve conduits.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Nervos Periféricos
/
Regeneração
/
Atenção
/
Células de Schwann
/
Ciclo Celular
/
Fibronectinas
/
Células PC12
/
Tenascina
/
Fator de Crescimento Neural
/
Fator Neurotrófico Derivado de Linhagem de Célula Glial
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Dental Anesthesia and Pain Medicine
Ano de publicação:
2017
Tipo de documento:
Artigo
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