Regulation of Tumor Necrosis Factor-alpha-induced Airway Mucin Production and Gene Expression by Carbenoxolone, Prunetin, and Silibinin / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 348-353, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-204137
ABSTRACT
BACKGROUND:
In this study, we tried to investigate whether carbenoxolone, prunetin, and silibinin affect tumor necrosis factor (TNF)-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells.METHODS:
Confluent NCI-H292 cells were pretreated with each agent (carbenoxolone, prunetin, and silibinin) for 30 min and then stimulated with TNF-alpha for 24 hours. The MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay, respectively.RESULTS:
Carbenoxolone, prunetin and silibinin inhibited the production of MUC5AC mucin protein induced by TNF-alpha; the 3 compounds also inhibited the expression of MUC5AC mucin gene induced by TNF-alpha.CONCLUSION:
This result suggests that carbenoxolone, prunetin and silibinin can inhibit mucin gene expression and production of mucin protein induced by TNF-alpha, by directly acting on airway epithelial cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Silimarina
/
Ensaio de Imunoadsorção Enzimática
/
Carbenoxolona
/
Expressão Gênica
/
Fator de Necrose Tumoral alfa
/
Células Epiteliais
/
Mucina-5AC
/
Isoflavonas
/
Mucinas
/
Necrose
Limite:
Humanos
Idioma:
Inglês
Revista:
Tuberculosis and Respiratory Diseases
Ano de publicação:
2010
Tipo de documento:
Artigo
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