The Protective Effect of Ischemic and Hypoxic Preconditioning on Hypoxic-ischemic Brain Injury in the Neonatal Rat: 1H Magnetic Resonance Spectroscopic Study / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 188-197, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-205492
ABSTRACT
BACKGROUND:
A brief episode of cerebral ischemia confers transient ischemic tolerance to a subsequent ischemic challenge. We examined the effect of ischemic and hypoxic preconditioning in the neonatal rat.METHODS:
Seven-day old Sprague-Dawley rat pups were divided into three groupscontrol (n = 53), ischemic preconditioning (n = 51), and hypoxic preconditioning (n = 48). For ischemic preconditioning, the right common carotid artery was occluded for 10 min. Rats in the hypoxic preconditioning group were kept under hypoxic (8% oxygen/92% nitrogen) conditions for 4h. Twenty-four hours after the preconditioning, rats from all groups were exposed to the right common carotid artery ligature, followed by 2.5 h of hypoxia. Lipid/N-acetyl aspartate (Lip/NAA) and lipid/creatine (Lip/Cr) ratios from 1H MR spectroscopy and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) were evaluated as measures of apoptosis 1 and 7 days after hypoxic-ischemic injury.RESULTS:
In the ischemic and hypoxic preconditioning groups, the Lip/NAA and Lip/Cr ratios and the numbers of TUNEL-positive cells were significantly lower than those in the control group (P < 0.05), but there were no significant differences between the two preconditioning groups.CONCLUSIONS:
These results suggest that ischemic and hypoxic preconditioning in the neonatal rat attenuate the apoptosis that is caused by hypoxic-ischemic brain injury.
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Índice:
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Assunto principal:
Encéfalo
/
Lesões Encefálicas
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Espectroscopia de Ressonância Magnética
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Isquemia Encefálica
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Ratos Sprague-Dawley
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Artéria Carótida Primitiva
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Apoptose
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Ácido Aspártico
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Precondicionamento Isquêmico
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Ligadura
Limite:
Animais
Idioma:
Coreano
Revista:
Korean Journal of Anesthesiology
Ano de publicação:
2006
Tipo de documento:
Artigo
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