Simple Purification of Adeno-Associated Virus-DJ for Liver-Specific Gene Expression
Yonsei Medical Journal
;
: 790-794, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-205734
ABSTRACT
Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediate safe expression of transduced genes for several months. The liver is a major organ of metabolism, and liver-specific expression of a gene can be an invaluable tool for metabolic studies. AAV-DJ is a recombinant AAV generated by the gene shuffling of various AAV serotypes and shares characteristics of AAV2 and AAV8. AAV-DJ contains a heparin-binding domain in its capsid, which suggests that a heparin column could be used for the purification of the AAV. Given that AAV-DJ has been only recently available, relatively little is known about the optimal preparation/purification and application of AAV-DJ. Here, we present a simple large-scale preparation method that can generate 3×10(13) viral particles for in vivo experiments and demonstrate liver-specific gene expression via systemic injection in mice.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Expressão Gênica
/
Capsídeo
/
Genoma Viral
/
Dependovirus
/
Proteínas do Capsídeo
/
Células Hep G2
/
Vetores Genéticos
/
Fígado
/
Camundongos Endogâmicos C57BL
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2016
Tipo de documento:
Artigo
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