Characterization About Vibrio alginolyticus Phage Isolated from Marine Products
Journal of the Korean Society for Microbiology
; : 81-90, 1997.
Article
em Ko
| WPRIM
| ID: wpr-206588
Biblioteca responsável:
WPRO
ABSTRACT
Two phages for the pathogenic V. alginolyticus were isolated from marine products. These 2 phages were examined temperature stability, pH stability, inactivation by UV irradiation, damage on restriction system of host cell, antibody production, structure protein analysis and western blotting assay. V. alginolyticus phages(VAPs) fomed plaques about 0.5 - 0.9mm in diameter and bands 50 - 60% in sucrose density gradient, VAPs were stable below 65'C, pH 5 - 10 and mostly inactivation by UV irradiation for 120sec. Latend period was 15 - 20 min. and burst size was 1.3 - 1.4 * 10 PFU/cell. Restriction system of V. alginolyticus isolated was mostly inactivated by 45C, 20min. heating. VAPs had 14 specific structural proteins and 5 proteins related to antibody production.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Sacarose
/
Bacteriófagos
/
Vibrio
/
Western Blotting
/
Vibrio alginolyticus
/
Coriolaceae
/
Temperatura Alta
/
Calefação
/
Concentração de Íons de Hidrogênio
/
Formação de Anticorpos
Idioma:
Ko
Revista:
Journal of the Korean Society for Microbiology
Ano de publicação:
1997
Tipo de documento:
Article