Cloning, expression and functional analysis of the duck Toll-like receptor 5 (TLR5) gene
Journal of Veterinary Science
;
: 37-46, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-206914
ABSTRACT
Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation factor 88, 2'-5'-oligoadenylate synthetase (OAS), and myxovirus resistance (Mx) genes from duck. dTLR5 mRNA was highly expressed in the bursa of Fabricius, spleen, trachea, lung, jejunum, rectum, and skin; moderately expressed in the muscular and glandular tissues, duodenum, ileum, caecum, and pancreas; and minimally expressed in the heart, liver, kidney, and muscle. DF-1 or HeLa cells transfected with DNA constructs encoding dTLR5 can activate NF-kappaB leading to the activation of interleukin-6 (IL-6) promoter. When we challenged ducks with a Herts33 Newcastle disease virus (NDV), mRNA transcription of the antiviral molecules Mx, Double stranded RNA activated protein kinase (PKR), and OAS was up-regulated in the liver, lung, and spleen 1 and 2 days post-inoculation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Especificidade da Espécie
/
2',5'-Oligoadenilato Sintetase
/
Vírus da Doença de Newcastle
/
RNA Mensageiro
/
Linhagem Celular
/
Regulação da Expressão Gênica
/
Clonagem Molecular
/
Patos
/
Receptor 5 Toll-Like
/
Fator 88 de Diferenciação Mieloide
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2015
Tipo de documento:
Artigo
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