Tumorigenesis after Injection of Lung Cancer Cell Line (SW-900 G IV) into the Pleural Cavity of Nude Mice / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 588-595, 2010.
Artigo
em Coreano
| WPRIM
| ID: wpr-207002
ABSTRACT
BACKGROUND:
Base on types of tumor, the types of expressed tumor is diverse and the difference in its expression rate is even more various. Due to such reasons an animal model is absolutely needed for a clinical research of lung cancer. The author attempted oncogenesis by cultivating a cell line of non-small cell carcinoma and then injecting it inside thoracic cavities of nude mice. The author conducted quantitative analyses of HER2/neu tumor gene - an epidermal growth factor receptor (EGFR) related to lung cancer, and TGF-beta1, which acts as a resistance to cell growth inhibition and malignant degeneration. In order to investigate achievability of the oncogenesis, histological changes and the expression of cancer gene in case of orthotopic lung cancer is necessary. MATERIAL ANDMETHOD:
Among 20 immunity-free male BALB/c, five nude mice were selected as the control group and rest as the experimental group. Their weights ranged from 20 to 25 gm (Orient, Japan). After injection of lung cancer line (SW900 G IV) into the pleural cavity of nude mice, They were raised at aseptic room for 8 weeks. HER2/neu was quantitatively analyzed by separating serum from gathered blood via chemiluminiscent immunoassay (CLIA), and immunosandwitch method was applied to quantitatively analyze TGF-beta1 SPSS statistical program (SPSS Version 10.0, USA) was implemented for statistical analysis. Student T test was done, and cases in which p-value is less than 0.05 were considered significant.RESULT:
Even after lung cancer was formed in the normal control group or after intentionally injected lung cancer cell line, no amplification of HER2/neu gene showed reaction. However, the exact quantity of TGF-beta1was 28,490+/-8,549 pg/mL, and the quantity in the group injected with lung cancer cell was 42,362+/-14,449 pg/mL, meaning 1.48 times highly significant (p<0.483). It proved that HER2/neu gene TGF-beta1had no meaningful interconnection.CONCLUSION:
TGF-beta1gene expressed approximately 1.48 times amplification in comparison to the control group. The amplification of TGF-beta1meant somatic recuperation inhibition mechanism due to carcinogenesis in nude mice was definitely working. It may be implemented as a quantitative analysis that allows early detection of lung cancer in human body.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pesos e Medidas
/
Imunoensaio
/
Camundongos Transgênicos
/
Linhagem Celular
/
Transformação Celular Neoplásica
/
Corpo Humano
/
Modelos Animais
/
Cavidade Pleural
/
Intenção
/
Genes Neoplásicos
Tipo de estudo:
Estudo prognóstico
/
Estudo de rastreamento
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
The Korean Journal of Thoracic and Cardiovascular Surgery
Ano de publicação:
2010
Tipo de documento:
Artigo
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