Effect of Trypsin on Physico-dynamic and Histological Changes after Decellularization of Bovine Pericardium / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 565-575, 2010.
Artigo
em Coreano
| WPRIM
| ID: wpr-207004
ABSTRACT
BACKGROUND:
Various decellularization methods have been studied in order to develop tissue graft which is less immunogenic and more durable. This study was performed to investigate the physico-dynamic and histological effect of trypsin pretreatment on decellularization protocols. MATERIAL ANDMETHOD:
Two groups of bovine pericardium specimen each underwent decellularization process based on SDS and Triton X-100 or N-lauroylsarcosinate and Triton X-100. Two more groups additionally underwent pretreatment with 0.1% Trypsin/0.1% EDTA. After decellularization process, mechanical tensile strength was tested, then iomechanical test of permeability and compliance was tested before and after fatigue test. Light microscopy and electron microscopy was performed to observe histological findings.RESULT:
There was no difference in mechanical tensile strength between groups, but permeability and compliance was decreased in trypsin pretreated groups. Light microscopic and electron microscopic findings revealed damage of the extracellular matrix in trypsin pretreated groups and in groups which underwent the fatigue test also.CONCLUSION:
Trypsin pretreatment in decellularizing process of bovine pericardium damages extracellular matrix and increases permeability and compliance of the bovine pericardium, but did not decrease tensile strength. Further studies are needed to use enzymatic treatments in decellularization protocols.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pericárdio
/
Permeabilidade
/
Sarcosina
/
Resistência à Tração
/
Engenharia Biomédica
/
Bioprótese
/
Microscopia Eletrônica
/
Tripsina
/
Ácido Edético
/
Octoxinol
Tipo de estudo:
Guia de Prática Clínica
Idioma:
Coreano
Revista:
The Korean Journal of Thoracic and Cardiovascular Surgery
Ano de publicação:
2010
Tipo de documento:
Artigo
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