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Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration
Korean Journal of Radiology ; : 194-198, 2002.
Artigo em Inglês | WPRIM | ID: wpr-207028
ABSTRACT

OBJECTIVE:

To compare the efficiency of intra-arterial, intraportal, and intravenous administration of cationic lipid emulsion/DNA complex, as used for gene transfer to rat liver. MATERIALS AND

METHODS:

DNA-carrier complex for the in-vivo experiment was prepared by mixing DNA and a cationic lipid emulsion. According to the administration route used (intra-arterial, intraportal, or intravenous), the animals were assigned to one of three groups. The heart, lung, liver, spleen and kidneys were removed and assayed for total protein and luciferase concentration.

RESULTS:

The cationic lipid emulsion/DNA complex used successfully transfected the various organs via the different administration routes employed. Luciferase activity in each organ of untreated animals was negligible. Liver luciferase values were significantly higher in the groups in which intra-arterial or intraportal administration was used.

CONCLUSION:

The intra-arterial or intraportal administration of cationic lipid emulsion/DNA complex is superior to intravenous administration and allows selective gene transfer to the liver.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: DNA / Estudo Comparativo / Cateterismo / Cátions / Ratos Sprague-Dawley / Técnicas de Transferência de Genes / Emulsões / Injeções Intra-Arteriais / Injeções Intravenosas / Lipídeos Limite: Animais Idioma: Inglês Revista: Korean Journal of Radiology Ano de publicação: 2002 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: DNA / Estudo Comparativo / Cateterismo / Cátions / Ratos Sprague-Dawley / Técnicas de Transferência de Genes / Emulsões / Injeções Intra-Arteriais / Injeções Intravenosas / Lipídeos Limite: Animais Idioma: Inglês Revista: Korean Journal of Radiology Ano de publicação: 2002 Tipo de documento: Artigo