Effects of Tyrosine Kinase Inhibitors on the Calcium-Dependent K+ Current in Rat Basilar Smooth Muscle Cells
Journal of Korean Neurosurgical Society
;
: 393-398, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-207127
ABSTRACT
OBJECTIVE:
Tyrosine kinase inhibitors may be useful in the management of cerebral vasospasm. It has not yet reported whether potassium channel plays a role in tyrosine kinase inhibitors-induced vascular relaxation of cerebral artery. This study is undertaken to clarify the role of potassium channel in tyrosine kinase inhibitors-induced vascular relaxation, and to investigate the effect of tyrosine kinase inhibitors on outward potassium currents in freshly isolated smooth muscle cells from rat basilar artery.METHODS:
The isolation of rat basilar smooth muscle cells was performed by special techniques. The whole cell currents were recoreded by whole cell patch clamp technique in freshly isolated smooth muscle cells from rat basilar artery.RESULTS:
In present study, genistein(n=10), tyrphostin A-23(n=10), A-25(n=10) 30microM into bath solution increased the amplitude of the outward K+ current which was completely blocked by large conductance calcium-activated potassium channel(BK(Ca)) blocker, iberiotoxin(0.1microM), and calcium chelator, BAPTA, in whole cell mode. In contrast, diadzein 30microM(n=10), inactive analogue of genistein, did not increase the amplitude of the outward K+ current.CONCLUSION:
Our results suggest tyrosine kinase inhibitors such as genistein, tyrphostin A-23 and A-25 increase the BK(Ca) channel activity in cerebral basilar smooth muscle cells, thereby contributing to the relaxation of cerebral artery.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Potássio
/
Relaxamento
/
Tirosina
/
Artéria Basilar
/
Banhos
/
Proteínas Tirosina Quinases
/
Canais de Potássio
/
Artérias Cerebrais
/
Cálcio
/
Genisteína
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Korean Neurosurgical Society
Ano de publicação:
2003
Tipo de documento:
Artigo
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