Effects of Transplantation with Marrow-Derived Mesenchymal Stem Cells Modified with Survivin on Renal Ischemia-Reperfusion Injury in Mice
Yonsei Medical Journal
; : 1130-1137, 2014.
Article
em En
| WPRIM
| ID: wpr-207148
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: To determine whether renal injury induced by ischemia-reperfusion (I/R) could be further improved by mesenchymal stem cells (MSCs) modified with survivin. MATERIALS AND METHODS: Lentiviral vectors were used to introduce the survivin gene into MSCs and the MSCs modified with survivin were transplanted into established mice models of renal I/R injury. Seven days later, serum creatinine (Scr) and blood urea nitrogen (BUN) were measured and the survival of MSCs was determined. Hematoxylin and eosin staining was used to assess renal pathological change. The expressions of hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) in kidney tissue were detected by western blot. RESULTS: Mice transplanted with survivin-modified MSCs demonstrated good renal function recovery with Scr and BUN decline close to normal levels and improvement of renal I/R injury repair. Additionally, the survival of transplanted MSCs modified with survivin was enhanced and the expression of HGF and bFGF in kidney tissue was increased. CONCLUSION: Our results demonstrated that MSCs engineered to over-express survivin could enhance their therapeutic effect on renal I/R injury in mice, probably via the improved survival ability of MSCs and increased production of protective cytokines in ischemic tissue.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Proteínas Repressoras
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Células da Medula Óssea
/
Traumatismo por Reperfusão
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Transplante de Células-Tronco Mesenquimais
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Proteínas Inibidoras de Apoptose
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Camundongos Endogâmicos C57BL
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Yonsei Medical Journal
Ano de publicação:
2014
Tipo de documento:
Article