Mutations of the Epidermal Growth Factor Receptor Gene in Triple-Negative Breast Cancer / 한국유방암학회지
Journal of Breast Cancer
;
: 150-159, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-207534
ABSTRACT
PURPOSE:
Epidermal growth factor receptor (EGFR) is considered a potential therapeutic target for anti-EGFR therapy in triple-negative breast cancer (TNBC). However, the frequency of EGFR gene mutation in TNBC is low and varies with ethnicity. This study aimed to investigate the incidence of EGFR gene mutation in TNBC.METHODS:
EGFR protein expression was evaluated by immunohistochemistry in tissue microarrays of 493 TNBC cases using four different primary antibodies, which included mutation-specific antibodies. For cases with an immunoreactivity level ≥1+, we performed pyrosequencing analysis for EGFR gene mutation. A case was considered mutation-positive when its mutation frequency minus its limit of detection (LOD) was >10%. Cases with mutation frequency higher than LOD were assessed for EGFR gene mutation status using the Cobas assay and by peptide nucleic acid-mediated polymerase chain reaction (PNA-clamping).RESULTS:
Among 493 TNBCs, 148 (30.0%) exhibited staining ≥1+ for EGFR, including 78 with 1+, 49 with 2+, and 21 with 3+. Positive EGFR expression (≥2+) was significantly associated with lymphovascular invasion (p=0.010), but not with overall survival (p=0.444) or disease-free survival (p=0.388). None of the 493 TNBCs harbored an EGFR gene mutation. Among 148 cases with an EGFR staining result ≥1+, five (3.4%) showed mutation frequencies (4.4%–10.9%) higher than LOD (2.6%–4.3%) in exons 19 (L747_P753>Q) or 21 (L858R and L861Q) as determined by pyrosequencing. However, Cobas and PNA-clamping failed to detect the presence of EGFR gene mutation in these five cases.CONCLUSION:
No activating mutation of EGFR gene of clinical significance was observed in 148 TNBC cases using three commercially available methods. Thus, EGFR gene mutation appears to be an extremely rare event in patients with TNBC.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias da Mama
/
Imuno-Histoquímica
/
Reação em Cadeia da Polimerase
/
Éxons
/
Incidência
/
Genes erbB-1
/
Intervalo Livre de Doença
/
Fator de Crescimento Epidérmico
/
Limite de Detecção
/
Taxa de Mutação
Tipo de estudo:
Estudo de incidência
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Breast Cancer
Ano de publicação:
2017
Tipo de documento:
Artigo
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