Molecular Identification of Taenia Tapeworms by Cox1 Gene in Koh Kong, Cambodia
The Korean Journal of Parasitology
;
: 195-197, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-207824
ABSTRACT
We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Teníase
/
Camboja
/
Proteínas de Helminto
/
Reação em Cadeia da Polimerase
/
Análise de Sequência de DNA
/
Primers do DNA
/
DNA de Helmintos
/
Taenia saginata
/
Taenia solium
/
Ciclo-Oxigenase 1
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2011
Tipo de documento:
Artigo
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