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Induction of Signal Transduction Pathway Genes in Dendritic Cells by Lipopolysaccharides from Porphyromonas gingivalis and Escherichia coli
Article em En | WPRIM | ID: wpr-209525
Biblioteca responsável: WPRO
ABSTRACT
Porphyromonas (P.) gingivalis lipopolysaccharide (Pg LPS) is the major pathogenic component of periodontal disease. In this study, we have attempted to determine the expression profiles of the signal transduction pathway genes induced by Pg LPS in comparison with Escherichia (E.) coli LPS (Ec LPS). DC2.4 cells were treated for two hours with 1 microg/ml of Pg LPS or 0.5 microg/ml of Ec LPS. The total RNA from these cells was then isolated and reverse-transcribed. Gene expression profiles were then analyzed with a signal transduction pathway finder GEArray Q series kit and significant changes in expression were confirmed by real-time PCR. The microarray results indicated that several genes, including Tnfrsf10b, Vcam1, Scyb9, Trim25, Klk6, and Stra6 were up-regulated in the DC2.4 cells in response to Pg LPS treatment, but were downregulated or unaffected by Ec LPS. Real-time PCR revealed that the expression of Trim25, Scyb9 and Tnfrsf10b was increased over the untreated control. Notably, Trim25 and Tnfrsf10b were more strongly induced by Pg LPS than by Ec LPS. These results provide greater insight into the signal transduction pathways that are altered by P. gingivalis LPS.
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Texto completo: 1 Índice: WPRIM Assunto principal: Doenças Periodontais / Células Dendríticas / RNA / Transdução de Sinais / Lipopolissacarídeos / Porphyromonas / Porphyromonas gingivalis / Escherichia / Escherichia coli / Reação em Cadeia da Polimerase em Tempo Real Idioma: En Revista: International Journal of Oral Biology Ano de publicação: 2010 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Doenças Periodontais / Células Dendríticas / RNA / Transdução de Sinais / Lipopolissacarídeos / Porphyromonas / Porphyromonas gingivalis / Escherichia / Escherichia coli / Reação em Cadeia da Polimerase em Tempo Real Idioma: En Revista: International Journal of Oral Biology Ano de publicação: 2010 Tipo de documento: Article