The neuroprotective effect of recombinant human erythropoietin via an antiapoptotic mechanism on hypoxic-ischemic brain injury in neonatal rats / 소아과
Korean Journal of Pediatrics
;
: 898-908, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-209661
ABSTRACT
PURPOSE:
The neuroprotective effects of erythropoietin (EPO) have been recently shown in many animal models of brain injury, including hypoxic-ischemic (HI) encephalopathy, trauma, and excitotoxicity; however, limited data are available for such effects during the neonatal periods. Therefore, we investigated whether recombinant human EPO (rHuEPO) can protect against perinatal HI brain injury via an antiapoptotic mechanism.METHODS:
The left carotid artery was ligated in 7-day-old Sprague-Dawley (SD) rat pups (in vivo model). The animals were divided into 6 groups normoxia control (NC), normoxia sham-operated (NS), hypoxia only (H), hypoxia+vehicle (HV), hypoxia+rHuEPO before a hypoxic insult (HE-B), and hypoxia+rHuEPO after a hypoxic insult (HE-A). Embryonic cortical neuronal cell culture of SD rats at 18 days gestation (in vitro model) was performed. The cultured cells were divided into 5 groups normoxia (N), hypoxia (H), and 1, 10, and 100 IU/mL rHuEPO-treated groups.RESULTS:
In the in vivo model, Bcl-2 expressions in the H and HV groups were lower than those in the NC and NS groups, whereas those in the HE-A and HE-B groups were greater than those of the H and HV groups. The expressions of Bax and caspase-3 and the ratio of Bax/Bcl-2 were in contrast to those of Bcl-2. In the in vitro model, the patterns of Bcl-2, Bax, and caspase-3 expression and Bax/Bcl-2 ratio were similar to the results obtained in the in vivo model.CONCLUSION:
rHuEPO exerts neuroprotective effect against perinatal HI brain injury via an antiapoptotic mechanism.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Encéfalo
/
Lesões Encefálicas
/
Artérias Carótidas
/
Células Cultivadas
/
Eritropoetina
/
Apoptose
/
Fármacos Neuroprotetores
/
Técnicas de Cultura de Células
/
Modelos Animais
/
Caspase 3
Limite:
Animais
/
Humanos
/
Gravidez
Idioma:
Inglês
Revista:
Korean Journal of Pediatrics
Ano de publicação:
2010
Tipo de documento:
Artigo
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