Proliferation of Corneal Endothelial Cells by Delivery of SV40 Large T Antigen
Journal of the Korean Ophthalmological Society
;
: 1331-1335, 2001.
Artigo
em Coreano
| WPRIM
| ID: wpr-209889
ABSTRACT
PURPOSE:
To determine whether the delivery of the SV40 large T-antigen is a feasible method for transiently inducing proliferation of corneal endothelial cells, we delivered liposome-protein complex into bovine corneal endothelial cells(BCEC).METHOD:
SV40 large T-antigen protein was introduced into BCEC and positive cells were identified by immunohistochemistry. Quiescent BCECs were double-labeled using BrdU as a measure of de novo DNA synthesis and the Ki-67 was detected by standard immunohistochemical methods.RESULT:
The treatment of quiescent BCECs with large T antigen caused an increase in BrdU incorporation and Ki-67 expression. It was tested by time-course study.CONCLUSION:
This finding suggests that liposome-mediated delivery of transforming proteins could be a method to transiently induce corneal endothelial cell proliferation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bromodesoxiuridina
/
DNA
/
Imuno-Histoquímica
/
Células Endoteliais
/
Proliferação de Células
/
Antígenos Virais de Tumores
Tipo de estudo:
Estudo prognóstico
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2001
Tipo de documento:
Artigo
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