Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi
Journal of Korean Medical Science
;
: 866-870, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-210701
ABSTRACT
Orientia tsutsugamushi, a causative pathogen of Scrub typhus, is a gram-negative intracellular bacterium. Outer membrane vesicles (OMVs) are produced from the membrane of bacteria and play many roles related to the survival of the pathogen. However, there have been no reports confirming whether O. tsutsugamushi indeed produce OMVs. O. tsutsugamushi boryong was cultured in ECV-304 cells for the purification of OMVs. Western blot analysis and immunoenrichment using anti-O. tsutsugamushi monoclonal antibody and electron microscopy were employed for identification and characterization of OMVs. We confirm the presence of OMVs derived from O. tsutsugamushi, and also found that those OMVs contain a major surface antigen of 56-kDa protein and variant immunogenic antigens.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Orientia tsutsugamushi
/
Tifo por Ácaros
/
Microscopia Eletrônica
/
Linhagem Celular
/
Membrana Celular
/
Vesículas Secretórias
/
Anticorpos Monoclonais
/
Antígenos de Bactérias
/
Antígenos de Superfície
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Korean Medical Science
Ano de publicação:
2015
Tipo de documento:
Artigo
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