The Study of the Cytotoxic Mechanism of Cisplatin in HeLa Cells / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 1989-1998, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-21097
ABSTRACT
Cis-diamminedichloroplatinum II (cisplatin) has been reported to induce cell death. However, the mechanism by which cisplatin is induced the apoptosis of cancer cells is still unclear. To evaluate the mechanistic insights of apoptosis by cisplatin, we tested the activities of apoptotic signaling pathway in HeLa cells. Apoptotic death of HeLa cells by cisplatin was confirmed by ladder-pattern fragmentation of genomic DNA. Cisplatin induced the activation of caspase-3 and 9 proteases in a time dependent manner. The caspase-3 protease activation and the cleavage of poly (ADP-ribose) polymerase (PARP) and procaspase-3 was demonstrated. We also showed that the expression of Bcl-2 and Bcl-xL was markedly decreased by the addition of cisplatin in HeLa cells. Moreover, expression of Fas and FasL proteins was increased by cisplatin. These data suggest that cisplatin triggers the activation of apoptotic signaling pathway in human cervical cancer, HeLa cells, via affects the expression of Fas, FasL and Bcl-2 families as well as triggers the activation of caspase-3 and -9 proteases.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptídeo Hidrolases
/
DNA
/
Células HeLa
/
Neoplasias do Colo do Útero
/
Cisplatino
/
Morte Celular
/
Apoptose
/
Caspase 3
/
Proteína Ligante Fas
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
2003
Tipo de documento:
Artigo
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