Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening
The Korean Journal of Parasitology
;
: 21-26, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-210971
ABSTRACT
Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes de Fusão
/
Anticorpos Anti-Helmínticos
/
Dados de Sequência Molecular
/
Biomarcadores Tumorais
/
Expressão Gênica
/
Clonagem Molecular
/
Análise de Sequência de DNA
/
Estruturas Animais
/
Dirofilaria immitis
/
Modelos Animais de Doenças
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2014
Tipo de documento:
Artigo
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