Changes in Hypothalamic Expressions of Natriuretic Peptide mRNA following Head-down Suspension in Rats
Korean Journal of Aerospace and Environmental Medicine
; : 7-12, 2003.
Article
em Ko
| WPRIM
| ID: wpr-211572
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: Head-down suspension (HDS) of rats has been used as a model for simulation of a microgravity environment. C-type natriuretic peptides (CNP) and atrial natriuretic peptide (ANP) are produced in the central nervous system, especially in hypothalamus, to complement their peripheral natriuretic effects. Therefore, this study investigated the changes in the central adaptations of hypothalamic ANP and CNP syntheses to 4 weeks of HDS in rats. METHODS: Unanesthetized, unrestrained, male Sprague-Dawley rats were subjected to either a horizontal position (control rats) or a -45 degrees head-down tilt using the tail-traction technique (HDS rats). We determined the hypothalamic syntheses of natriuretic peptides as an expression of ANP and CNP mRNA. The expression of natriuretic peptide mRNA was measured by reverse transcription-polymerase chain reaction with [32P]-dCTP following 4 weeks of HDS in the hypothalamus of control and HDS rats. RESULTS: After 4 weeks of HDS, the expression of ANP mRNA showed a decreasing trend in the hypothalamus of HDS rats. In contrast with ANP, CNP mRNA expression was significantly (p<0.01) increased in the hypothalamus of HDS rats. There were different changes in the hypothalamic CNP and ANP mRNA expressions of HDS rats compared with that of the control rats.CONCLUSION: These results represent that the hypothalamic syntheses of natriuretic peptides are differently responded and the role of CNP is augmented to compensate for the decrement of ANP action in the central nervous system following 4 weeks of HDS.
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Índice:
WPRIM
Assunto principal:
Ausência de Peso
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Proteínas do Sistema Complemento
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RNA Mensageiro
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Sistema Nervoso Central
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Fator Natriurético Atrial
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Ratos Sprague-Dawley
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Decúbito Inclinado com Rebaixamento da Cabeça
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Peptídeos Natriuréticos
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Natriuréticos
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Hipotálamo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
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Male
Idioma:
Ko
Revista:
Korean Journal of Aerospace and Environmental Medicine
Ano de publicação:
2003
Tipo de documento:
Article