Analysis of Cellular Changes Resulting from Forced Expression of Dickkopf-1 in Hepatocellular Carcinoma Cells / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 30-36, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-212924
ABSTRACT
PURPOSE:
Recent studies have shown that Dickkopf-1 (DKK-1) is overexpressed in some tumors, including hepatocellular carcinoma. However, the role of increased DKK-1 in these tumors is not known. In this study, the DKK-1 expression in hepatocellular carcinoma (HCC) cell lines was evaluated and the effect of DKK-1 overexpression in HCC cell lines was studied. MATERIALS ANDMETHODS:
The expression of DKK-1 in hepatocellular carcinoma cell lines was evaluated by RT-PCR. Stable cell lines that overexpressed DKK-1 were established. Cell growth, adhesion, migration and invasion assays were performed.RESULTS:
RT-PCR analysis showed that 5 out of 8 HCC cell lines expressed DKK-1. The forced expression of DKK-1 suppressed the growth of cells and increased the population of cells in the sub-G1 phase. In addition, DKK- 1 reduced the cellular adhesion capacity to collagen type I and fibronectin, and it increased migratory capacity. However, overexpression of DKK-1 did not increase the invasion capacity of the HCC cell line.CONCLUSION:
Collectively, our data suggest that overexpression of DKK-1 affects the biology of HCC cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Biologia
/
Adesão Celular
/
Linhagem Celular
/
Fibronectinas
/
Apoptose
/
Carcinoma Hepatocelular
/
Colágeno Tipo I
Idioma:
Inglês
Revista:
Cancer Research and Treatment
Ano de publicação:
2007
Tipo de documento:
Artigo
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