A PCR-RFLP method for the detection of activated H-ras oncogene with a point mutation at codon 12 and 61
Yonsei Medical Journal
;
: 371-379, 1996.
Artigo
em Inglês
| WPRIM
| ID: wpr-213092
ABSTRACT
To investigate the incidence of the H-ras gene activation in bladder tumor and the feasibility of using urinary washout samples for screening, a series of 33 human bladder tumors and their preoperatively collected urinary washout samples were screened using a mutant specific PCR-RFLP (polymerase chain-restriction fragment length polymorphism) to detect a point mutation of the H-ras gene. Five tumors were found to harbor H-ras mutations where two tumors had a glycine to valine (G-->T) change in codon 12 and three tumors had a glutamine to lysine (C-->A) change in codon 61, respectively. Moreover, we could also detect the same point mutations of the H-ras gene in corresponding urine washout samples. The incidence of H-ras mutation in Korean bladder cancer was estimated at approximately 15.2%. In conclusion, a mutant specific PCR-RFLP method for the detection of H-ras gene mutation is useful for screening or postoperative follow-up of bladder tumor due to its simplicity and high specificity even in urinary samples.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias da Bexiga Urinária
/
Polimorfismo de Fragmento de Restrição
/
Códon
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Carcinoma de Células de Transição
/
Regulação da Expressão Gênica
/
Reação em Cadeia da Polimerase
/
Genes ras
/
Mutação Puntual
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
1996
Tipo de documento:
Artigo
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