Apoptosis in Vero cells infected with Akabane, Aino and Chuzan virus
Journal of Veterinary Science
;
: 251-254, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-213962
ABSTRACT
Akabane, Aino and Chuzan virus are arthropod-borne (arbo)viruses mainly associated with reproductive failures in cattle. We investigated apoptosis in Vero cells (C-1586) infected with Akabane, Aino and Chuzan virus. The fragmentation of chromosomal DNA was simultaneously detected with the progress of cytopathic effect from 48 hr to 72 hr post infection, depending on viruses. Although the treatment of cycloheximide blocked apoptosis in Vero cells infected with three viruses, actinomycin D did not prevent DNA oligomerization, thus indicating that de novo viral protein synthesis is critical for viral apoptosis. In addition, the activation of caspase-3 was also detected in Vero cells by indirect fluorescent assay. From the present results, it is of future interest whether apoptotic characteristics of these viruses are related to pathogenecity in vivo.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células Vero
/
Bunyaviridae
/
Chlorocebus aethiops
/
Apoptose
/
Orbivirus
/
Caspases
/
Efeito Citopatogênico Viral
/
Dactinomicina
/
Ativação Enzimática
/
Caspase 3
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2005
Tipo de documento:
Artigo
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