Expressed sequence tags analysis of Blattella germanica
The Korean Journal of Parasitology
;
: 149-156, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-215235
ABSTRACT
Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Dados de Sequência Molecular
/
Sequência de Bases
/
Alinhamento de Sequência
/
Blattellidae
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Etiquetas de Sequências Expressas
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2005
Tipo de documento:
Artigo
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