Analysis of Microsatellite Instability in Ovarian Epithelial Cancer
Korean Journal of Pathology
;
: 380-386, 2007.
Artigo
em Coreano
| WPRIM
| ID: wpr-215313
ABSTRACT
BACKGROUND:
The aim of this study was to clarify the incidence and role of microsatellite instability (MSI) in sporadic ovarian epithelial cancers (OEC). We investigated the MSI status and mismatch repair (MMR) protein expression in OEC.METHODS:
MSI was examined by fluorescence- based polymerase chain reaction using five NCI panel markers (BAT25, BAT26, D2S123, D5S346 and D17S250) in 46 cases of OEC. Immunohistochemistry (IHC) for hMLH1 and hMSH2 was performed.RESULTS:
Seven cases (15.2%) exhibited high-frequency MSI (MSIH), one exhibited low-frequency MSI (MSI-L), and the remaining 38 demonstrated microsatellite stability (MSS). MSI-H in OEC was not associated with histologic grade, FIGO stage, tumor size, mitoses or histologic type. Loss of expression of either hMLH1 or hMSH2 was observed in 4 of the 7 (59.3%) MSI-H cases, whereas 4 of the 39 (10.3%) MSI-L or MSS tumors revealed loss of expression of MMR proteins. The sensitivity and specificity of immunohistochemistry for hMLH1 and hMSH2 were 57.1% and 89.7%.CONCLUSIONS:
Our data suggest that a genetic defect in the MMR system might play a role in the carcinogenesis of a minor subset of sporadic OEC however, immunohistochemical testing for hMLH1 and hMSH2 cannot accurately determine microsatellite instability status in OEC.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Ovarianas
/
Imuno-Histoquímica
/
Reação em Cadeia da Polimerase
/
Incidência
/
Sensibilidade e Especificidade
/
Repetições de Microssatélites
/
Instabilidade de Microssatélites
/
Reparo de Erro de Pareamento de DNA
/
Carcinogênese
/
Mitose
Tipo de estudo:
Estudo diagnóstico
/
Estudo de incidência
/
Estudo prognóstico
Idioma:
Coreano
Revista:
Korean Journal of Pathology
Ano de publicação:
2007
Tipo de documento:
Artigo
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