Comparison of 3 Automated Immunoassays for Detection of Anti-Hepatitis A Virus Immunoglobulin M in a Tertiary Care Hospital
Annals of Laboratory Medicine
;
: 121-124, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-216012
ABSTRACT
Three automated immunoassay kits for anti-Hepatitis A Virus (HAV) IgM-Architect, (Abbott Laboratories, USA), Elecsys (Roche Diagnostics, Germany), and ADVIA Centaur (Siemens Healthcare Diagnostics Inc., USA)-were compared. We included 178 consecutive samples, for which an anti-HAV IgM test was requested at Seoul National University Hospital from September 2009 to January 2010. Reviewing of medical records, reverse transcription (RT)-PCR for HAV RNA, or total anti-HAV assay were performed on 16 (9.0%) samples with discrepant results. The percent agreements (kappas) of the Architect and ADVIA Centaur, Architect and Elecsys, and ADVIA Centaur and Elecsys kits were 96.6% (0.91), 96.6% (0.92), and 97.8% (0.94), respectively. Eight out of 16 discrepant samples showed gray-zone values in Architect but were nonreactive in the others. Slightly earlier seroconversion was suspected in Elecsys. The 3 assays showed comparable performances with excellent agreements in a tertiary care hospital setting.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Atenção Terciária à Saúde
/
Imunoglobulina M
/
RNA Viral
/
Imunoensaio
/
Estudos Retrospectivos
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vírus da Hepatite A
/
Hepatite B
/
Hospitais Universitários
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
Limite:
Humanos
Idioma:
Inglês
Revista:
Annals of Laboratory Medicine
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS