Lactoferrin Stimulates Mouse Macrophage to Express BAFF via Smad3 Pathway
Immune Network
;
: 84-88, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-216359
ABSTRACT
B cell-activating factor belonging to the TNF family (BAFF) is primarily expressed by macrophages and stimulates B cell proliferation, differentiation, survival, and Ig production. In this study, we explored the effect of lactoferrin (LF) on BAFF expression by murine macrophages. We determined the level of BAFF expression at the transcriptional and protein levels using RT-PCR and ELISA, respectively. LF markedly enhanced BAFF expression in mouse macrophages at both the transcriptional and protein levels. Overexpression of Smad3/4 further increased LF-induced BAFF transcription while DN-Smad3 abolished the LF-induced BAFF expression. These results demonstrate that LF can enhance BAFF expression through Smad3/4 pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Proliferação de Células
/
Fator de Crescimento Transformador beta1
/
Lactoferrina
/
Macrófagos
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Immune Network
Ano de publicação:
2012
Tipo de documento:
Artigo
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