Evaluation of the VIDAS CDAB Kits for the Detection of the Clostridium difficile Toxins A and B / 대한임상미생물학회지
Korean Journal of Clinical Microbiology
;
: 107-111, 2008.
Artigo
em Coreano
| WPRIM
| ID: wpr-217984
ABSTRACT
BACKGROUND:
Since the emergence of variant Clostridium difficile strains that fail to produce detectable toxin A, diagnostic kits targeted to detect toxin A only showed a considerable rate of false negative results. The aim of this study was to evaluate a toxins A and B (toxins A/B) detection kit recently marketed in Korea, and to compare toxin positive rates before and after introduction of the new kit.METHODS:
The results of 5,783 toxin A assays performed during the 7-year period from 2001 through 2007 were analyzed and compared them to the toxins A/B assay data of 519 samples obtained from January to June 2008 in a university hospital. An enzyme-linked fluorescent immunoassay for toxins A/B (VIDAS C. difficile Toxin A & B, bioMerieux SA, France VIDAS CDAB) and PCR for toxin genes A/B were performed directly in 102 stool samples from hospitalized patients.RESULTS:
The positive rates of toxin A assays tended downward annually from 2001 to 2007 (16.3%, 17.8%, 13.9%, 11.4%, 13.8%, 8.2%, and 5.8%, respectively), but increased to 12.1% in 2008 after changing to the toxin A/B detection kit. The concordant rate of the VIDAS CDAB kit with the PCR method was 82.4%. Compared to the PCR method, the sensitivity and specificity of the toxin A/B kit were 60.7% and 90.5% respectively.CONCLUSION:
Testing kits for C. difficile toxin A only could result in a misdiagnosis more frequently than the testing kit for toxins A/B. The sensitivity of the newly launched toxin A/B detection kit from bioMerieux SA needs to be improved, but it showed a good specificity
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Imunoensaio
/
Reação em Cadeia da Polimerase
/
Clostridioides difficile
/
Técnicas Imunoenzimáticas
/
Sensibilidade e Especificidade
/
Clostridium
/
Erros de Diagnóstico
/
Coreia (Geográfico)
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Coreano
Revista:
Korean Journal of Clinical Microbiology
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS