Effects of irradiation on TGF-beta1 mRNA expression and calcific nodule formation in MC3T3-E1 osteoblastic cell line / 대한구강악안면방사선학회지
Korean Journal of Oral and Maxillofacial Radiology
;
: 125-132, 2008.
Artigo
em Coreano
| WPRIM
| ID: wpr-218758
ABSTRACT
PURPOSE:
To investigate the effects of irradiation on transforming growth factor beta1 (TGF-beta1) mRNA expression and calcific nodule formation in MC3T3-E1 osteoblastic cell line. MATERIALS ANDMETHODS:
Cells were cultured in alpha-minimum essential medium (alpha-MEM) supplemented with 10% fetal bovine serum and antibiotics. When the cells reached the level of 70-80% confluence, culture media were changed with alpha-MEM supplemented with 10% FBS, 5 mM beta-glycerol phosphate, and 50 microgram/mL ascorbic acid. Thereafter the cells were irradiated with a single dose of 2, 4, 6, 8 Gy at a dose rate of 1.5 Gy/min. The expression pattern of TGF-beta1 mRNA, calcium content and calcific nodule formation were examined on day 3, 7, 14, 21, 28, respectively, after the irradiation.RESULTS:
The amount of TGF-beta1 mRNA expression decreased significantly on day 7 after irradiation of 4, 6, 8 Gy. It also decreased on day 14 after irradiation of 6, 8 Gy, and decreased on day 21 after irradiation of 8 Gy. The amount of calcium deposition decreased significantly on day 7 after irradiation of 4, 8 Gy (P.0.01) and showed a decreased tendency on day 14, 21 after irradiation of 4, 6, 8 Gy. The number of calcific nodules was decreased on day 7 after irradiation of 4, 8 Gy.CONCLUSION:
Irradiation with a single dose of 4, 6, 8 Gy influences negatively the bone formation at the molecular level by affecting the TGF-beta1 mRNA expression that was associated with proliferation and the production of extracellular matrix in MC3T3-E1 osteoblastic cell line.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Compostos Orgânicos
/
Osteoblastos
/
Osteogênese
/
Ácido Ascórbico
/
RNA Mensageiro
/
Linhagem Celular
/
Cálcio
/
Meios de Cultura
/
Matriz Extracelular
/
Fator de Crescimento Transformador beta1
Idioma:
Coreano
Revista:
Korean Journal of Oral and Maxillofacial Radiology
Ano de publicação:
2008
Tipo de documento:
Artigo
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