Modified Method of Multilocus Sequence Typing (MLST) for Serotyping in Salmonella Species
Journal of Bacteriology and Virology
;
: 314-318, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-218816
ABSTRACT
Multilocus sequence typing (MLST) of Salmonella is useful method for replacing serotyping using antisera but is limited by difficulties associated with in polymerase chain reaction (PCR). We optimized the PCR reaction, especially annealing temperature and extension time (94degrees C for 2 min; 40 cycles at 94degrees C for 30 sec, 56.8degrees C for 1 min, 72degrees C for 2 min; and 72degrees C for 10 min). The degradation of PCR product by thermostable nucleases was inhibited by using template DNAs treated proteinase K or purified by a commercialized preparation kit. The resulting modified MLST was used as accurate and fast typing method.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Salmonella
/
DNA
/
Sorotipagem
/
Reação em Cadeia da Polimerase
/
Endopeptidase K
/
Tipagem de Sequências Multilocus
/
Soros Imunes
Idioma:
Inglês
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2015
Tipo de documento:
Artigo
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