Involvement of the CXC chemokines Mig and IP-10 in response to M. bovis BCG in mice
Journal of the Korean Society for Microbiology
; : 117-127, 2000.
Article
em Ko
| WPRIM
| ID: wpr-219060
Biblioteca responsável:
WPRO
ABSTRACT
The non-ELR-containing CXC chemokines Mig and IP-10 have been shown to function as chemotactic cytokines for activated T lymphocytes. In this study, we examined the potential involvement of Mig and IP-10 in antimycobacterial response of mice immunized or infected with M. bovis BCG. The accumulation of Mig and IP-10 mRNA in resident peritoneal monocytes (RPMPHI) was slightly reduced by stimulation with vBCG, and the degree was greater for 24 hr culture even though IFN-gamma was added. Expression of Mig, IP-10, and IFN-gamma in 24 hr delayed-type hypersensitivity (DTH) response was stronger in vBCG-immune mice than in the non-immune. The increase of DTH measured by foot-pad thickness appears to be clearly related to the levels of chemokines Mig and IP10 messages and those of IFN-gamma and IL-12. Stimulation with vBCG for 2 days decreased or completely dropped the levels of Mig message in non-immune or immune splenocytes, respectively, whereas IP-10 message was slightly decreased in 2 days culture. Moreover, messages for IL-12 (p40) showed similar kinetics for Mig. The levels of Mig and IP-10 mRNA during the course of infection with BCG were not readily changed in lungs, livers, and spleens from BCG-infected mice. Although there was no obvious changes of Mig and IP-10 messages in the target organs during infection process, we found that the infection progressed over the first 3 wk before being contained by the emerging immune response suggested from detectable amount of IFN-gamma mRNA around this time. In view of selectivity of chemokines Mig and IP-10 for activated T cells, these data suggest that chemokine Mig and IP-10, especially in collaboration with IL-12 and IFN-gamma, may play a role as T cell recruiters in immune response against mycobacterial infection.
Texto completo:
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Índice:
WPRIM
Assunto principal:
Baço
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RNA Mensageiro
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Cinética
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Monócitos
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Linfócitos T
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Interleucina-12
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Quimiocinas
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Comportamento Cooperativo
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Quimiocinas CXC
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Hipersensibilidade
Limite:
Animals
Idioma:
Ko
Revista:
Journal of the Korean Society for Microbiology
Ano de publicação:
2000
Tipo de documento:
Article